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Saturday, 10 April 2021

All Detail about RT PCR Test

All Detail about RT PCR Test


Collection centre level entry of patient details undergoing RT-PCR test COVID19

The app is a hand held tool for the Medical Staff at Sample Collection Centres spread across the country. The sample collection facility will be sending the  sample for various type of specimen to ICMR labs conducting the RT-PCR test for confirmation of COVID19. Advance intimation is being shared through the app with ICMR . The user has to read the terms and conditions carefully before using the app. On successful saving of any sample, the collection centre user can also view the PDF listing collection details.

PCR game crushing the toughest diseases with your molecular biology skills

 All Detail about RT PCR Test

RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation currently available. Compared to the two other commonly used techniques for quantifying mRNA levels, Northern blot analysis and RNase protection assay, RT-PCR can be used to quantify mRNA levels from much smaller samples. In fact, this technique is sensitive enough to enable quantitation of RNA from a single cell.

This article first discusses the advantages of real-time RT-PCR compared to end-point methods. This discussion is followed by a description of the different methods for quantitating gene expression by real-time RT-PCR with respect to the different chemistries available, the quantitation methods used and the instrumentation options available. Subsequently, the “traditional” methods of quantitating gene expression by RT-PCR, i.e. end-point techniques, are presented.



Why Real-Time RT-PCR?

Over the last several years, the development of novel chemistries and instrumentation platforms enabling detection of PCR products on a real-time basis has led to widespread adoption of real-time RT-PCR as the method of choice for quantitating changes in gene expression. Furthermore, real-time RT-PCR has become the preferred method for validating results obtained from array analyses and other techniques that evaluate gene expression changes on a global scale.

To truly appreciate the benefits of real-time PCR, a review of PCR fundamentals is necessary. At the start of a PCR reaction, reagents are in excess, template and product are at low enough concentrations that product renaturation does not compete with primer binding, and amplification proceeds at a constant, exponential rate. The point at which the reaction rate ceases to be exponential and enters a linear phase of amplification is extremely variable, even among replicate samples, but it appears to be primarily due to product renaturation competing with primer binding (since adding more reagents or enzyme has little effect). At some later cycle the amplification rate drops to near zero (plateaus), and little more product is made.

Test your PCR knowledge with Thermo Fisher Scientific’s lab game – PCR Quest – where you travel from lab to lab crushing the world’s toughest diseases. Perfect science game for adults. 

Plan your moves by matching 3 or more viruses and bacteria in a row to crush a disease. Use your lab supply boosters—dry ice, petri dishes and isolated DNA to overcome those extra elusive bugs. The more diseases you crush the higher your score. 

Matching Viruses and Bacteria:

When four viruses or bacteria of the same color form a match and the direction of the last swap is horizontal this special virus is created. When this new virus forms a match, it will crush all the blocks in that same row. Four viruses of the same color form a match in a vertical pattern a special vertical virus is created as well. And when that virus forms a match it will crush all the blocks in that same vertical column. 

zero (plateaus), and little more product is made.



What is RT-PCR test

RT-PCR (Reverse Transcription Polymers Chain Reaction) Test. In addition, rapid antigen and antibody tests are also done. If the rapid antigen test is positive, then the person is positive for corona. But if the test is negative and symptoms of corona still appear, it becomes necessary to have an RT-PCR test.

This test provides information about the virus in a person. In which the ribonucleic acid (RNA) of the virus is examined. Making RNA from deoxyribonucleic acid (DNA) is called transcription.

RT PCR TEST વિશે ડીટેઇલ માહિતી અહિંથી વાંચો

What is CT value

According to the Indian Council of Medical Research (ICMR), the RT-PCR test measures SRS-CoV-2 and then measures its positivity based on the cycle threshold (CT).

If you match 5 viruses of the same color together a special crushing virus is formed that eliminates all other surrounding viruses. 

Chain Reactions:

Forming matching viruses or bacteria in an “L” or “T” shaped pattern created a chain reaction. This is identified by a multicolored object with the game. When this is swiped with any adjacent virus or bacteria a chain reaction is formed and eliminates those viruses.

RT-PCR ટેસ્ટ વિડીયો અહીંથી જુઓ

Special Bacteria:

Tiles are not affected by gravity and cannot be moved or swapped to make a match. These include thermal blast, extraction and cycler.

PCR Powers: 

Are available from the controls station located at the bottom of the game screen.

DNA Strands:

Challenges take place where you are asked to collect DNA strands. These DNA strands are collected when they reach the bottom of the game board.

Lives:

You have a set number of lives to the play the game. You can gain more lives by earning more coins.

Coins:

Earn coins by completing lab goals and interacting with PCR educational content found throughout the game. 

Stars:

Earn stars by gaining as many points as possible at each lab you visit.

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